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SmartPReP 2 system makes concentrating autolgous stem cells simple, easy, and fast. The SmartPReP BMAC procedure packs were specifically desinged to concentrate autolgous stem cells from bone marrow aspirate. The unique automated dual-spin and decanting process coupled with self-calibrating disposables ensures consistent and reproducible end product.

 

Mean % Yield

Mean Number of Cells

Platelets x 106/ml

73.9 + 17.1

794 x 106 + 429

Mononuclear Cells x106/ml

70.2 + 29.9

376 x 106 + 79

CD34+ Cells x106/ml

74.6 + 13.7

9.02 x 106 + 4.3

Colony Form Units-Fibroblast/cm3*

3,040 + 1,251

60,800 + 29,200

* An aggregate of cells containing more than 50 Fibroblasts was scored as a colony.

Performance

There has been a lot of excitement regarding the use of bone marrow derived stem cells; especially in orthopedics as a potential to eliminate the complications associated with iliac crest graft harvesting. Consequently, it is critical to choose a technology platform that is not only documented to reliably concentrate all the stem and accessory cells but also concentrates these cells without diminishing their biologic potential.

As an example, selective retention techniques are less than optimal processing systems. The process routinely fails to capture a large number of cells present in the bone marrow aspirate. Furthermore, the end product is limited in the types of cells, molecules and proteins it is able to recover from bone marrow and return to the patient.

Unlike these selective retention techniques, the SmartPReP BMAC system is designed to concentrate a buffy coat from whole bone marrow aspirate. Because a buffy coat is a physiological fraction, it contains the total compliment of all stem cell types as well as platelets and other soluble factors that play a role in skeletalgenesis.

 

 

 

Data on File

Bone Marrow Aspirate Vol., ml

Total Nucleated Cells
x106/ml

Platelets
x106/ml

Mononuclear Cells
x106/ml

CD34+
x103/ml

* Colony Forming Units-F/cm3

Total Progenitor Cells

Selective Retention

60

35.6

126.6

9.6

412

841

7,575

BMAC

120

197.2

1,182.6

43.8

1,384

2,662

50,596

* Hernigou, et al, (Percutaneous Autologous Bone Marrow Grafting - Influence of the Number and Concentration of Stem Cells, JBJS), showed healing of nonunions in patients receiving > 1,500 CFU-F/cm3

Quality

The method used for concentrating stem cells has a direct impact on the stem cells. In order to deliver enough progenitor cells, the stem cells not only need to be concentrated but concentrated in such a way as to leave them viable and functional. Stem cells concentrated using the SmartPReP BMAC system meet these critical criteria.

When concentrated BMAC stem cells and commercially available hMSC's (used as a control) are exposed to Mesenchymal Stem Cell culture media, the BMAC culture demonstrated a retained capacity for cell division and maintain normal morphology when compared to the control hMSC cells.

When concentrated BMAC stem cells and commercially available hMSC's are exposed to an osteogenic differentiation medium (ODM), the BMAC cells and control cells retained their ability and potential for osteoblastic differentiation.

 

 

Bright areas represent new deposition elements

Finally, when concentrated BMAC stem cells are exposed to Von Kossa silver stains, the BMAC cells demonstrated osteoblastic differentiation as evidenced by the vacuoles with black stain indicating deposited calcium.

 

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